There is an urgent need for more physiologically relevant cell culture methods to guide compound selection in pre-clinical stages of the drug development pipeline.

Promotor: Prof.dr. B. van de Water

Promotor: Prof.dr. H.P. Spaink, Co-promotor: Prof.dr. A.H. Meijer

Promotor: B. van de Water

The challenge of achieving fast quantification in metabolomics is the presence of severe matrix effects during the MS analysis of complex samples.

We have studied shape with a particular focus on the zebrafish model system. The shape is an essential appearance of the phenotype of a biological specimen and it can be used to read out a current state or response or to study gene expression.

Promotor: Prof.dr. T. Hankemeier

Metabolomics has the potential to play a pivotal role in understanding disease onset and progression, and ultimately personalized treatments. One of its major challenges is its large-scale implementation, which is necessary to deal with the high variability of the metabolome. In this work we have developed…

Promotor: T. Schmidt

De Microscopy Unit huisvest, onderhoudt en coördineert vrijwel alle microscopieapparatuur van het IBL. De beschikbare apparatuur varieert van conventionele licht- en fluorescentiemicroscopen tot confocale laser- en elektronenmicroscopen. Er is bovendien apparatuur beschikbaar voor histologie, met inbegrip…

Single-Molecule Microscopy (SMM) techniques constitute a group of powerful imaging tools that enable researchers to study the dynamic behavior of individual molecules.

Pharmaceutical companies, governments and the general public have become increasingly aware that animal models used in drug testing lack vital aspects to serve as an accurate representation of human biology. As models of the human body should become more physiologically relevant, animal models no longer…

In this thesis we investigated the ability of two-photon multifocal microscopy for single-molecule microscopy in live cells and organisms.

In this thesis we use Josephson and noise scanning tunneling microscopy for the study of conventional, unconventional (iron-based) and disordered superconductors. On the one hand, Josephson scanning tunneling microscopy allows us to directly visualize the superfluid density with high spatial resolut…

There are many interpretations of quantum mechanics, and ultimately experiments are needed to verify or falsify these interpretations.

In this thesis, we describe the latest advances in SQUID-detected Magnetic Resonance Force Microscopy (MRFM).

Promotor: B. van de Water

This thesis presents insights from our study of various correlated electron systems with a scanning tunneling microscope (STM). In ordinary metals, electron-electron interactions exist, but get substantially screened due to the sheer number of electrons.

Electron microscopy has become an extremely important techniquein a wide variety of elds.

The dissertation describes TEM experiments on heterogeneous catalysts.

Traditional drug discovery approaches have been hampered by (in vitro) cell-culture models that poorly represent the situation in the human body.

Promotor: Prof.dr. J.N. Kok, Co-promotor: F.J. Verbeek

Promotor: Prof.dr. R.M.H. Merks

This thesis described the development of novel scanning tunneling microscopy techniques to investigate strongly correlated electronic states in quantum matter.

In surface science there is great effort to move from studying simple, flat model surfaces in vacuum to investigating more complex model catalysts in gas environments (in situ). This thesis gives three examples of such studies using microscopy and spectroscopy.

Het doel van deze faciliteit is om het biomedisch onderzoek te versnellen door toegang te bieden tot geavanceerde licht- en elektronenmicroscopie en door het delen van expertise over monstervoorbereiding, beeldvormingsmodaliteiten, gegevensopslag en beeldanalyse.

Promotor: Prof.dr. J.M. van Ruitenbeek, Prof.dr. R.M. Tromp

In this thesis, we show how MRFM can usefully contribute to the field of condensed-matter.

Promotores: Prof.dr. H.P. Spaink & Prof.dr. P.C.W. Hogendoorn Co-promotor: Dr. M.J.M. Schaaf

n this PhD thesis, the recombination of different atomic lattices in stacked 2D materials such as twisted bilayer graphene is studied. Using the different possibilities of Low-Energy Electron Microscopy (LEEM), the domain forming between the two atomic layers with small differences is studied.

Promotores: Prof.dr. C.U. Keller, Prof.dr. H.C. Gerritsen

Promotor: T.H. Oosterkamp

Hoe kun je met robots en automatische herkenning van microscopische beelden uitzonderlijk snel het effect van medicijnen testen?

The thesis introduces three methods for high-dimensional prediction problems in the biomedical field. The methods make use of empirical and variational Bayes in the estimation.

Promotor: Prof.dr. J.N. Kok

To study how planetary systems come into existence we study much younger systems still in formation.

More than 5,000 exoplanets have been found over the past couple of decades. These exoplanets show a tremendous diversity, ranging from scorching hot Jupiters, common super-Earths, to widely separated super-Jupiters on the planet/brown dwarf boundary.

Promotores: Prof.dr. P.C.W. Hogendoorn & Prof.dr. H.P. Spaink Co-promotor: Dr. B.E. Snaar-Jagalska

This thesis investigates the relations between metabolism and anatomy through the use of Magnetic Resonance Imaging (MRI).

Promotor: A.P.IJzerman Co-promotor: A. Bender

The main aim of this thesis was to unravel the signaling and regulatory networks that drive tumor cell migration during breast cancer metastasis.

The zebrafish is a promising vertebrate model organism in early drug discovery and development.

Outflows are crucially important for the gas budget and evolution of luminous star-forming galaxies and AGNs, with observed mass outflow rates of the same order as the star formation rate. Greater star formation and black hole growth lead to more intense feedback and outflows, resulting in self-regulated…

Drug-induced liver injury (DILI) is one of the main reasons for drug attrition during pre-clinical and clinical phases of drug development as well as for drug withdrawal post-marketing.

From surface science towards industrial practice using high-pressure scanning tunneling microscopy.

This thesis aims to demonstrate how the achromatic nature and design flexibility of liquid-crystal optics can be used to improve high-contrast imaging instruments to facilitate detailed exoplanet characterization.

The sequence-dependence of biomolecular interactions involving nucleic acids and proteins is essential for numerous processes inside the cell. Insights into the underlying molecular mechanisms have been obtained using various biochemical and biophysical methods on two different levels — bulk and sin…

Understanding the formation and evolution of planetary systems is one of the most fundamental challenges in astronomy. To directly image and study young exoplanets and the circumstellar disks they form from, dedicated high-contrast imaging instruments are built.

This thesis describes the implementation and on-sky demonstration of high-resolution integral-field spectroscopy for extreme adaptive optics systems that are used to find and characterize exoplanets.

This thesis is devoted to an in-depth examination of the various effects of disorder in the cuprate high-temperature superconductors.