The aim of this thesis is to study cell-cell interactions and the development of an assay to explore and quantify the exchange of membrane compounds.

Despite being the object of intense study, embryonic development has been difficult to model due to a number of reasons. First, complex tissues can be comprised of many cell types, of which we probably only know a subset.

In this thesis, we describe the potential of cell mechanical phenotyping for immune cell characterization, diagnosis, drug testing, as well as treatment of disease.

Mechanical interactions between cells and their environment play an important role in many biological processes.

Promotor: Prof.dr. J. Kuiper

The emergence of complex diseases resulting from abnormal cell-cell signaling and the spread of infectious diseases caused by pathogens are significant threats to humanity. Unraveling the dynamic mechanisms underlying cell-cell signaling and infectious disease spreading is crucial for effective disease…

Cardiovascular disease is a major global burden and atherosclerosis is the main underlying pathological process.

There is an urgent need for more physiologically relevant cell culture methods to guide compound selection in pre-clinical stages of the drug development pipeline.

This work describes the use of click-to-release chemistry to get spatiotemporal control over immunocytokine activity. Until now, immunocytokines (cytokines coupled to a tumor-targeting-moiety) remained active throughout the body, being able to bind their respective receptors, causing mild to severe…

The bacterial cell wall is a nearly universal structure that offers protection and gives the cell its shape. However, environmental stressors, such as cell wall-targeting antibiotics and hyperosmotic conditions, can induce bacteria to shift to a wall-deficient state. It is unknown whether the lack of…

Epithelial-mesenchymal plasticity (EMP) and tumor cell migration play an important role in cancer progression, and an improved understanding of the mechanisms underlying these concepts is essential for developing new targeted approaches.

There is a need for alternative methods to replace, reduce and refine (3R) animal experimentation. Combining experimental data from high-throughput in vitro studies with in silico modeling is a promising approach to unravel the effect of chemicals on living cells and to gain a better understanding of…

A new type of tripodal squaramide-based supramolecular hydrogels is developed and studied.

In this project of the Center for Public Values & Ethics (CPVE) ongoing research is conducted on the history of advice to Dutch central government by so-called State committees from 1814 to 2014. State committees have been installed by Dutch central government since 1814 to provide expert advice, research…

During embryonic growth, cells proliferate, differentiate, and collectively migrate to form different tissues at the right position and time in the body.

Almost all bacteria are enveloped by a cell wall that provides cellular protection.

In this research project, we aimed to obtain more insight in the role of mast cells in the immune driven disorders rheumatoid arthritis and atherosclerosis.

Aspergillus niger is an important industrial producer of organic acids and enzymes producing large amounts of spent fungal biomass.

Traditional drug discovery approaches have been hampered by (in vitro) cell-culture models that poorly represent the situation in the human body.

In this thesis, we have studied cell images from two types of cells, including pollen grains and the immune cells, neutrophils. These images are captured using a bright field microscope and a confocal microscope.

Acute cardiovascular syndromes, including myocardial infarction or stroke, are the principal cause of death in the Western society. The main underlying pathology of cardiovascular diseases is atherosclerosis, which is caused by the accumulation of lipids and inflammatory cells in the vessel wall, in…

This thesis focuses on two processes involved in fighting infections: metabolism and immune cell motility and navigation.

Wil jij jouw eigen vragen en ideeën vertalen naar wetenschappelijke onderzoeksprojecten? Worden jouw interesses geprikkeld door technologie? Durf jij wetenschap kritisch te benaderen en haar grenzen te verleggen?

This thesis describes the design and synthesis of fragments of various cell wall carbohydrates of the Streptococcus species, including the branched Group B-specific antigen (GBC) of Group B Streptococcus, glycerol phosphate (GroP) modified group A carbohydrate (GAC), and the O-acetylated type 1 capsular…

Waarom Molecular Science & Technology in Leiden? Je wordt niet chemicus óf technoloog, maar beide! De opleidingsvideo vertelt je er alles over.

Het beste van Leiden en Delft komt samen in de bachelor opleiding Life Science & Technology. Benieuwd naar het programma? Bekijk de video.

Deze tweejarige masteropleiding wordt aangeboden door het Leiden Institute of Chemistry en heeft een focus op technologische en fundamentele kennis en expertise. Tijdens de opleiding doe je onderzoek naar de processen van de levende cel met fundamenteel wetenschappelijke én toegepaste technologie. Je…

De onderzoeksgroep Business, Technology & Public Policy van de afdeling Bedrijfswetenschappen van Universiteit leiden houdt zich bezig met het onderzoeken van de dynamische wisselwerking tussen ‘nieuwe’ technologieën en de verschillende belanghebbenden die hiermee interacteren, waaronder consumenten,…

Het doet het Europa Instituut genoegen aan te kondigen dat op 21 november 2016 de 5e druk is verschenen van het handboek “EU State Aids” (31 hoofdstukken, 1500 pagina’s).

In this thesis, the focus lies on studying glucocorticoid receptor dynamics in living cells with the aim of understanding how this transcription factor finds its DNA target sites to regulate transcription.

Promotor: W.Th.F. den Hollander Co-Promotor: F.M. Spieksma

The way organisms develop from the initial single-cellular state to a complex final assembly like the human body, and how the final body is maintained throughout life, is one of the greatest mysteries and it’s understanding one of the biggest scientific challenges.

De faciltieit ontwikkelt en gebruikt, high-throughputmethoden toe voor genomisch onderzoek.

CONCLUSION: This study demonstrates that human macrophages can be induced to exert direct anti-tumor activity against osteosarcoma cells. Our observation that the induction of macrophage anti-tumor activity by L-MTP-PE required IFN-gamma may be of relevance for the optimization of L-MTP-PE therapy in…

Quantum Information Science & Technology (QIST) encompasses the understanding, design, construction and investigation of quantum information processing systems, such as quantum computers, quantum communication networks, and quantum sensors.

Promotie

In order to gain a better insight into the basis of the rapid jasmonic acid (JA)-mediated stress response, an integrated approach using a targeted high-resolution mass spectrometry-based (HRMS) platform, was conducted to monitor the metabolism of JA using the model system of cell suspension cultures…

Onder de gezamenlijke titel ‘Scarcity and the State’ verschenen onlangs twee Engelstalige boeken over de verdeling van schaarse publieke rechten (Intersentia 2016). Paul Adriaanse (UL), Frank van Ommeren (VU), Willemien den Ouden (UL) en Johan Wolswinkel (Tilburg University) voerden de redactie. Aan…

Carbohydrates are present on the surface of bacteria making them suitable antigen candidates for vaccine development. This thesis deals with the synthesis of two carbohydrate-based components; the capsular polisaccharide of S. aureus type 5 and teichoic acids from staphilococci and enterococci speci…

DNA-profilering op een crime-scene, DNA-chips voor het opsporen van ziektegerelateerde genen, wasmiddelen met enzymen die bij hoge temperatuur nog werkzaam zijn, bacteriën die helpen bij het zuiveren van afvalwater en het gebruik van gentherapie als medicijn.

Hoe kunnen we chemische processen nog efficiënter maken? Hoe ziet ons gebruik van brandstoffen eruit in de toekomst? Kunnen we door middel van de kennis van de chemie van de mens nog betere medicijnen maken? Zullen we ooit ziektes compleet kunnen uitbannen?

Bij de bacheloropleiding Life Science and Technology leer je de biologie van de cel doorgronden, en leer je ingewikkelde problemen op een efficiënte en systematische manier oplossen.

Streptomyces are multicellular, Gram-positive bacteria in the phylum of actinobacteria which produce a high amount of bioactive natural products of which the expression is tightly coordinated with the life cycle.

Bacteria have the ability to alter their morphology in order to adapt to changing environments.

Bij de bacheloropleiding Molecular Science & Technology aan de Universiteit Leiden ontwikkelen studenten een sterke theoretische basis in de scheikunde en doen ze kennis van de technologische toepassingen op. Daarnaast leren studenten een bijdrage te leveren aan vraagstukken op het gebied van duurzaamheid,…

Promotores: Prof.dr. G.F.A. Kersten, W. Jiskoot, Co-promotor: J-P. Amorij

Cyber agent technology en de WIV is een project dat in opdracht van Tracks Inspector, een bedrijf dat innovatieve software ontwikkelt, wordt uitgevoerd.

Synthetic hydrogels that mimic the natural extracellular matrix in the biophysical and biochemical cues it provides to cells are in high demand, however the cell phenotypes as they are observed in vivo in numerous cases have yet to be attained.

Cytotoxic T-cells (CTLs) are involved in the clearance of viruses and killing of tumor cells.