Promotor: Prof.dr. H.S. Overkleeft

Less than 1 in 10 drug candidates that enter phase 1 clinical trials actually gets approved for human use.

Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt

To this day, all cyclophellitol-based inhibitors and ABPs have been close analogues of their natural substrate counterparts. As a result, these probes showed high selectivity towards their target glycosidases.

In the last decades, activity-based protein profiling (ABPP) has emerged as a powerful chemical tool that may aid the ever-challenging drug discovery process.

Glycosidases are important enzymes in the turnover of polysaccharides and glycoconjugates, and are involved in a range of human pathologies including genetic disorders such as Gaucher and Pompe disease, but also in various cancers.

Promotor: Prof.dr. T. Hankemeier

Promotores: H.S. Overkleeft, G.A. van der Marel, Co-promotor: B.I. Florea

Promotores: Prof.dr. H.S. Overkleeft, Prof.dr. G.A. van der Marel

The adenosine receptors are proteins that reside in the extracellular membranes of cells. Activation of adenosine receptors plays a role in many physiological and pathological processes, such as immune responses and cancers.

Overkleeft

Laatste technologische ontwikkelingen op het gebied van datamining en profilering.

Promotor: H.S. Overkleeft, Co-Promotor: B.I. Florea

In de afgelopen jaren is het verzamelen en verwerken van tal van gegevens aanzienlijk toegenomen. Met behulp van nieuwe technologieën zoals data mining wordt getracht kennis te halen uit verzamelde gegevens. Vaak wordt gezocht naar profielen die eigenschappen of risico's van bepaalde groepen beschrijven.…

Acute cardiovascular syndromes, including myocardial infarction or stroke, are the principal cause of death in the Western society. The main underlying pathology of cardiovascular diseases is atherosclerosis, which is caused by the accumulation of lipids and inflammatory cells in the vessel wall, in…

Gedupeerden zijn een rechtszaak begonnen tegen etnisch profileren door de Koninklijke Marechaussee. Peter Rodrigues, hoogleraar immigratierecht zegt dat etniciteit nooit het belangrijkste criterium mag zijn om iemand staande te houden en verwijst naar een eerdere uitspraak van de Hoge Raad.

Het was al duidelijk dat de Belastingdienst jarenlang met zwarte lijsten werkte met daarop namen van mensen met een, volgens de fiscus, hoog frauderisico. Maar rapporten van onderzoeksbureau PwC maken duidelijk hoe stelselmatig de Belastingdienst discrimineerde bij het bepalen wie er op die lijst th…

Glycoside hydrolases (glycosidases/GHs) are widely abundant enzymes in all kingdoms of life and are important biocatalysts that catalyze the hydrolysis of glycosidic linkages in oligo/polysaccharides, glycoproteins and glycolipids with tremendous efficiency

Van der Woude en Rodrigues hebben een artikel geschreven in het Nederlands Juristenblad over proactieve politiecontrole naar etnische afkomst en nationaliteit.

This thesis describes the development and optimization of the first molecular tools to study the enzyme PLA2G4E.

Growth hormone profiles are pulsatile and highly variable between individuals, limiting the implementation of mathemathical models to quantify an individual's secretion.

The chemical synthesis of inhibitors and probes targeting endo-glycosidases.

Glycosidases are essential in fundamental biological processes and are responsible for the degradation of most (oligo)saccharides, glycolipids and glycoproteins.

Promotor: H.S. Overkleeft, J. Neefjes, Co-promotor: M. van der Stelt

This thesis aimed to investigate the impact of exposure dynamics, relative contributions of ENPs(particle) and ENPs(ion), and dosing regimens on the toxicity of ENPs varying in different physico-chemical properties, on the composition and functioning of soil microbial communities.

Vitamin A or retinol is essential in embryonic development, the visual cycle and the immune system.

Neuraminidases are enzymes that cleave glycosidic linkages of sialic acid. These enzymes are involved in influenza infections as well as in many cellular processes in mammals and micro-organisms.

CONSENT (Consumer sentiment regarding privacy on user generated content services in the digital economy) is a research project funded by the European Union under the 7th Framework Programme. The question central to this research is whether recent changes to consumer and commercial practices developed…

1. To what extent do metallic NPs added to soil extractions change the activity, abundance, or community composition of microbes? 2. How do the effects of metallic NPs on soil microbes differ from the effects of the ions shedding from corresponding NPs?

This thesis describes the use of an activity-based proteomics method to study the endocannabinoid system.

This thesis describes the synthesis and biochemical evaluation of a variety of cyclophellitol based activity-based probes and inhibitors targeting various endo- and exo-acting retaining glycosidases. In the last two decades a variety of probes and inhibitors for (hemi)cellulose degrading enzymes have…

Promotor: Prof.dr. B. van de Water

Lysosomal acid glucosylceramidase (GBA1) is a lysosomal enzyme that degrades glucolipids with its main substrate being glucosylceramide (GlcCer). Defects in the GBA1 gene lead to glycosphingolipidosis Gaucher disease (GD), in which the hydrolysis of GlcCer is impaired and therefore, it accumulates in…

Synthesis and application of chemical biology tools to study immunomodulatory signaling lipids.

Plants produce an astonishing variety of secondary metabolites (SMs) which are thought to play vital roles in the fitness of plants through ecological interactions.

Loopbaan en solliciteren

Migratie en criminaliteit staan maatschappelijk volop in de schijnwerpers. Binnen het instituut voor Strafrecht en Criminologie heeft onderzoek op dit terrein zich de laatste jaren sterk ontwikkeld. Het begrip Crimmigratie staat daarbij centraal.

Large parts of the research described in this Thesis aims at the development of oligopeptide-masked toxins and their in situ immunoproteasome-mediated activation.

Pseudomonas Aeruginosa is a Gram-negative bacterium which can form biofilms, increasing its resistance against antibiotics and the host immune system. Polysaccharides are an integral part of this biofilm, one of these polysaccharides is called Psl. PslG is a glycosidase, able to cleave this polysaccharide,…

The research profile ‘Asian Modernities and Traditions’ (AMT) aims to raise the strength and visibility of research, teaching and dissemination of Asian studies at Leiden University.

Accurate prediction of the unbound drug concentration-time profile at the CNS target site is crucial for the assessment of the right drug concentration-effect relationship. PBPK models have supported the PK prediction of the CNS target sites and the translation of PK data between species and between…

Lysosomal glycosidases are acid hydrolases that fragment glycoconjugates in lysosomes.

Dark matter is one of the biggest mysteries of the Universe. Its properties cannot be explained with the known laws of physics and elementary particles.

Welke impact hebben discussies over en bewezen beschuldigingen van etnisch profileren op organisaties en de (grens)politieagenten die op operationeel niveau werken? Gedurende een periode van drie jaar (november 2023 – november 2026) zal dit innovatieve transdisciplinaire onderzoeksproject zich richten…

Promotores: H.S. Overkleeft, G.A. van der Marel, Co-Promotor: R.G. Boot

Op 23 oktober 2017 geeft mr.dr.ir. Bart Custers, hoofd onderzoek bij eLaw, het centrum voor recht en digitale technologie van de Universiteit Leiden, een keynote lecture over big data en recht op de International Conference on Big Data Research (ICBDR 2017) in Osaka, Japan.