In this thesis, we describe the potential of cell mechanical phenotyping for immune cell characterization, diagnosis, drug testing, as well as treatment of disease.

During embryonic growth, cells proliferate, differentiate, and collectively migrate to form different tissues at the right position and time in the body.

Despite being the object of intense study, embryonic development has been difficult to model due to a number of reasons. First, complex tissues can be comprised of many cell types, of which we probably only know a subset.

The aim of this thesis is to study cell-cell interactions and the development of an assay to explore and quantify the exchange of membrane compounds.

De master Biology is een samenwerking tussen het Instituut Biologie Leiden, Naturalis en het Centrum voor Milieuwetenschappen Leiden waardoor het programma een brede en diverse scope heeft. De opleiding biedt zeven specialisaties, waarvan vier researchspecialisaties die nauw verbonden zijn met het onderzoek…

Quantitative biology means exploiting mathematical modelling and computer simulation as a tool to gain additional insights into the meaning of experimental data. Discover in this minor when and how to apply these tools, in a multidisciplinary setting.

Mechanical interactions between cells and their environment play an important role in many biological processes.

The deeper understanding of an organism's pathology is important for developing treatments. Over centuries of systematic research, clinical researchers have demonstrated that the more information they acquire about the cellular properties and their organisation in the tissue, the better they can understand…

Promotor: Prof.dr. J. Kuiper

Het chemische biologie-onderzoek bij het Leids Instituut voor Chemisch Onderzoek (LIC) is gericht op het begrijpen van biologische processen op moleculair niveau, om zo onze kennis te vergroten over gezondheid en ziekte van de mens.

De afdeling richt zich op het verbeteren van het wetenschappelijk begrip in de relatie tussen mens en natuur. Hoe beïnvloedt menselijk handelen (via bijvoorbeeld klimaatverandering, landgebruiksverandering en de emissie van mogelijk toxische verbindingen) de natuur in het algemeen en de biodiversiteit…

In deze opleiding identificeer je evolutionaire veranderingen over de tijd en bestudeer je de oorsprong en het behoud van biologische variatie in de Tree of Life.

Na afronding van deze opleiding beschik je over een eerstegraads onderwijsbevoegdheid en ben je goed voorbereid op een carrière als docent Biologie in het voortgezet onderwijs.

Cardiovascular disease is a major global burden and atherosclerosis is the main underlying pathological process.

This work describes the use of click-to-release chemistry to get spatiotemporal control over immunocytokine activity. Until now, immunocytokines (cytokines coupled to a tumor-targeting-moiety) remained active throughout the body, being able to bind their respective receptors, causing mild to severe…

Wiskunde en Natuurwetenschappen

Van systeembiologische modellering een centrale pijler van het onderzoek in de biologie maken.

Epithelial-mesenchymal plasticity (EMP) and tumor cell migration play an important role in cancer progression, and an improved understanding of the mechanisms underlying these concepts is essential for developing new targeted approaches.

Deze opleiding biedt de mogelijkheid om biologie-onderzoek te combineren met kennis over ondernemerschap en ervaring in het bedrijfsleven.

A new type of tripodal squaramide-based supramolecular hydrogels is developed and studied.

There is a need for alternative methods to replace, reduce and refine (3R) animal experimentation. Combining experimental data from high-throughput in vitro studies with in silico modeling is a promising approach to unravel the effect of chemicals on living cells and to gain a better understanding of…

LUMC Technology Focus Area (TFA) Data Analytics is een samenwerkingsverband van verschillende onderzoeksgroepen in het LUMC die nieuwe kwantitatieve en computationele methodologie ontwikkelen voor biomedisch en klinisch onderzoek.

The emergence of complex diseases resulting from abnormal cell-cell signaling and the spread of infectious diseases caused by pathogens are significant threats to humanity. Unraveling the dynamic mechanisms underlying cell-cell signaling and infectious disease spreading is crucial for effective disease…

Promotores: H.S. Overkleeft, G.A. van der Marel, Co-Promotor: R.G. Boot

In deze opleiding ligt de focus op het informeren van het algemene publiek over belangrijke wetenschappelijke onderwerpen en hoe je deze communicatie wetenschappelijk onderbouwt.

Almost all bacteria are enveloped by a cell wall that provides cellular protection.

In this research project, we aimed to obtain more insight in the role of mast cells in the immune driven disorders rheumatoid arthritis and atherosclerosis.

Aspergillus niger is an important industrial producer of organic acids and enzymes producing large amounts of spent fungal biomass.

Deze opleiding biedt de mogelijkheid om onderzoek op moleculair, cellulair en organismaal niveau te verenigen, maar biedt ook de mogelijkheid om je te specialiseren op één van deze niveaus.

Traditional drug discovery approaches have been hampered by (in vitro) cell-culture models that poorly represent the situation in the human body.

In this thesis, we have studied cell images from two types of cells, including pollen grains and the immune cells, neutrophils. These images are captured using a bright field microscope and a confocal microscope.

Acute cardiovascular syndromes, including myocardial infarction or stroke, are the principal cause of death in the Western society. The main underlying pathology of cardiovascular diseases is atherosclerosis, which is caused by the accumulation of lipids and inflammatory cells in the vessel wall, in…

There is an urgent need for more physiologically relevant cell culture methods to guide compound selection in pre-clinical stages of the drug development pipeline.

Balsaminaceae is a diverse plant family characterized by a huge floral morphological diversity.

This thesis focuses on two processes involved in fighting infections: metabolism and immune cell motility and navigation.

De afdeling Environmental Biology (CML-EB) houdt zich bezig met vraagstukken rondom biodiversiteit en natuurlijke hulpbronnen op het platteland in westerse en niet-westerse gebieden.

The bacterial cell wall is a nearly universal structure that offers protection and gives the cell its shape. However, environmental stressors, such as cell wall-targeting antibiotics and hyperosmotic conditions, can induce bacteria to shift to a wall-deficient state. It is unknown whether the lack of…

This thesis describes the design and synthesis of fragments of various cell wall carbohydrates of the Streptococcus species, including the branched Group B-specific antigen (GBC) of Group B Streptococcus, glycerol phosphate (GroP) modified group A carbohydrate (GAC), and the O-acetylated type 1 capsular…

Promotor: H.S. Overkleeft, Co-Promotor: B.I. Florea

CONCLUSION: This study demonstrates that human macrophages can be induced to exert direct anti-tumor activity against osteosarcoma cells. Our observation that the induction of macrophage anti-tumor activity by L-MTP-PE required IFN-gamma may be of relevance for the optimization of L-MTP-PE therapy in…