268 zoekresultaten voor “protein” in de Publieke website
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Synthesis of well-defined ADP-Ribosylated biomolecules
Promotor: G. A. van der Marel, Co-promotor: D. V. Filippov
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'Q-wires': Synthesis, electrochemical properties and their application in electro-enzymology
An objective of this research was to achieve direct, well-defined and non-rate-limiting electron transfer between respiratory enzymes and the electrode surface by means of 'Q-wires'.
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Osteosarcoma: searching for new treatment options
Promotores: B. van de Water; P. Hogendoorn; J. Bovée Co-Promotor: E.H.J. Danen
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Topological decoding of biomolecular fold complexity
Biological polymers, including proteins and the genome, undergo folding processes crucial for their proper functioning. Even slight changes in the folding structure of these biopolymers can have significant implications, leading to the development of various pathological conditions, such as neurodegenerative…
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Transforming data into knowledge for intelligent decision-making in early drug discovery
Promotor: A.P.IJzerman Co-promotor: A. Bender
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The development of molecular tools for investigating NAD+ metabolism and signalling
Nicotinamide adenine dinucleotide (NAD+) is the substrate used for the introduction of the ubiquitous and highly dynamic PTM in which either one or multiple adenosine diphosphate ribose (ADPr) moieties are covalently attached to a nucleophilic side chain of an specific amino acid in the target protein…
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The role of the Bone Morphogenetic Protein Pathway in Colorectal Cancer
Promotie
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Using cryo-EM methods to uncover structure and function of bacteriophages
Bacteriophages, or phages for short, are the most abundant biological entity in nature. They shape bacterial communities and are a major driving force in bacterial evolution.
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Discovery of BUB1 kinase inhibitors for the treatment of cancer
The spindle-assembly checkpoint (SAC) is a safety mechanism which secures accurate chromosome segregation during mitosis.
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PI3K signaling and adherens junctions in invasive lobular breast cancer
Invasive lobular carcinoma (ILC) is the second most common type of breast cancer.
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Electrical and magnetic properties of ferritin: electron transport phenomena and electron paramagnetic resonance
Ferritin is a spherical metalloprotein, capable of storing and releasing iron in a controllable way. It is composed of a protein shell of about 12 nm and within its cavity, iron is stored in a mineral form.
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Integrating cellular and tissue dynamics with cell fate decisions through computational modeling
There is a need for alternative methods to replace, reduce and refine (3R) animal experimentation. Combining experimental data from high-throughput in vitro studies with in silico modeling is a promising approach to unravel the effect of chemicals on living cells and to gain a better understanding of…
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Formulation of influenza T cell peptides: In search of a universal influenza vaccine
Promotores: Prof.dr. G.F.A. Kersten, W. Jiskoot, Co-promotor: J-P. Amorij
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Dynamics of TNFalpha signaling and drug-related toxicity
In previous studies at our laboratory it was demonstrated that drug exposure of HepG2 cells can lead to an altered TNFα-induced NF-κB oscillatory phenotype, concurrent with a synergistically increased sensitivity for TNFα-induced apoptosis.
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Time is of the essence: investigating kinetic interactions between drug, endogenous neuropeptides and receptor
Promotor: A.P. IJzerman Co-promotor: L.H. Heitman
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Bioorthogonal Labeling Tools to Study Pathogenic Intracellular Bacteria
In this thesis, bioorthogonal chemistry is combined with correlative light-electron microscopy to selectively label and study pathogenic intracellular bacteria within the host immune cell.
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Discovery of novel inhibitors to investigate diacylglycerol lipases and α/β hydrolase domain 16A
Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt
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Nuclear magnetic resonance force microscopy at millikelvin temperatures
Promotor: T.H. Oosterkamp
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Key innate immune components controlling intracellular infection
Promotor: H.P. Spaink, Co-Promotor: A.H. Meijer
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Image analysis for gene expression based phenotype characterization in yeast cells
Promotores: T.H.W. Bäck, A. Plaat, Co-promotor: F.J. Verbeek
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Structural characterization of the cell envelope of Actinobacteria under changing environments
Bacteria have the ability to alter their morphology in order to adapt to changing environments.
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Synthesis and applications of cell wall glycopolimer fragments from Staphilococci and Enterococci
Carbohydrates are present on the surface of bacteria making them suitable antigen candidates for vaccine development. This thesis deals with the synthesis of two carbohydrate-based components; the capsular polisaccharide of S. aureus type 5 and teichoic acids from staphilococci and enterococci speci…
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A semisynthetic peptide-metalloporphyrin responsive matrix for artificial photosynthesis
This thesis is concerned with the constitution of chiral responsive matrix assemblies that may undergo light-driven conformational changes and self-select vibrations to develop vibronic states for driving semi-classical coherent transfer with nearly 100% yield in a lossless NCAP process.
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Intradermal delivery of nanoparticulate vaccines using coated and hollow microneedles
In summary, the collective results described in this thesis show that nanoparticulate vaccines can be delivered intradermally by coated and hollow microneedles and evoke antigen-specific immune responses.
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Novel Immune Cell-Based Therapies for Atherosclerosis
Promotor: Prof.dr. J. Kuiper
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Chemical Synthesis of Fragments of Streptococcal Cell Wall Polysaccharides
This thesis describes the design and synthesis of fragments of various cell wall carbohydrates of the Streptococcus species, including the branched Group B-specific antigen (GBC) of Group B Streptococcus, glycerol phosphate (GroP) modified group A carbohydrate (GAC), and the O-acetylated type 1 capsular…
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Role of integrin adhesions in cellular mechanotransduction
Promotor: B. vd Water, T. Schmidt, Co-Promotor: E.H.J. Danen
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Advances in GPCR Modeling Evaluated by the GPCR Dock 2013 Assessment: Meeting New Challenges
Source: Structure , Volume 22, Issue 8, pp. 1120-1139 (2014)
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Computational modeling of angiogenesis : from matrix invasion to lumen formation
Promotor: Roeland M.H. Merks
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Bioorthogonal chemistry to unveil antigen processing events
The research described in this thesis focused on the use of bioorthogonal antigens to investigate immunological processes in antigen presenting cells. Bioorthogonal antigens are antigenic proteins produced through recombinant expression in a methionine auxotrophic E. coli strain.
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Unraveling the auxin mechanism in 2,4-D induced somatic embryogenesis in Arabidopsis thaliana
Promotor: P. J. J. Hooykaas, Co-promotor: R. Offringa
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New polymyxin antibiotics for old problems: addressing nephrotoxicity and resistance
Polymyxins are clinically used antibiotics, discovered in mid-20th century. Once abandoned due to excessive nephrotoxicity, they are now used increasingly to address infections caused by multi-drug resistant Gram-negative bacteria.In this thesis, we describe the development and synthesis of analogues…
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Design and synthesis of metal-based chemotherapeutic agents for targeted DNA interactions or DNA repair pathway modulation
The research presented in this thesis explores the chemotherapeutic potential of metal-based compounds as chemotherapy agents, with an initial focus on the synthesis and DNA interaction studies of platinum and palladium compounds utilizing the [Pt(bapbpy)]2+ scaffold. The study identifies intercalation…
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Phase separation in lipid-based nanoparticles: exploring the nano-bio interface
This doctoral thesis is an effort to understand how lipid phase-separation induced by diacylglycerol analogues in lipid-based nanoparticles affects their in vivo behavior, leading to specific nanoparticle-protein communications and selective cell targeting.
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Systems pharmacology of the amyloid cascade
According to the amyloid cascade hypothesis, accumulation of beta-amyloid (Aβ) peptides initiates the pathological cascade in Alzheimer's disease (AD).
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Applications of Natural Deep Eutectic Solvents to Extraction and Preservation of Biomolecules
The recently introduced nature-originated deep eutectic solvents, so-called natural deep eutectic solvents (NADES) are considered as truly green solvents, which composed of natural ingredients found abundantly in organisms.
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Evaluation of the zebrafish embryo as an alternative model for hepatotoxicity testing
Promotor: Prof.dr. B. van de Water, Co-promotores: L.T.M. van der Ven, A.S. Kienhuis
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Omics data integration with genome-scale modelling of dopaminergic neuronal metabolism
Parkinson's disease is the second most common neurodegenerative disease in the world. One of its symptoms is the loss of dopaminergic neurons in the substantia nigra pars compacta.
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The role of the Arabidopsis AHL15/REJUVENATOR gene in developmental phase transitions
This thesis describes the functional analysis of the Arabidopsis AHL15 gene.
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Single-molecule fluorescence in sequence space
The sequence-dependence of biomolecular interactions involving nucleic acids and proteins is essential for numerous processes inside the cell. Insights into the underlying molecular mechanisms have been obtained using various biochemical and biophysical methods on two different levels — bulk and sin…
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Bioengineering and biophysics of viral hemorrhagic fever
Viral hemorrhagic fever (VHF) is a group of acute diseases caused by highly infectious viruses including Ebola, Lassa, Dengue viruses. Its high mortality rate poses high risk to public health, however, studies on VHF have been hampered due to the non-availability of proper models and incomplete knowledge…
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Quantitative protein mass spectrometry for kidney injury biomarker translation towards the clinical laboratory
Promotie
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Targeting MHC-I related proteins for cancer diagnosis and therapy
Promotie
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Illuminating N-acylethanolamine biosynthesis with new chemical tools
In this thesis, the discovery and optimization is described of chemical tools to study the N-acylethanolamine (NAE) biosynthetic pathway.
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Preclinical validation of putative targets in cardiovascular and metabolic disease
Cardiovascular disease is the number one cause of death worldwide. The most important risk factor for developing this disease is high cholesterol levels in the blood.
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Discovery of Reversible Monoacylglycerol Lipase Inhibitors
Monoacylglycerol lipase (MAGL) is the principal enzyme responsible for hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-AG). MAGL inhibition provides several potential therapeutic opportunities, including anti-nociceptive, anti-inflammatory and anti-cancer activity.
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Towards a single-molecule FRET study of Frauenfelder's nonexponential rebinding of CO in myoglobin
Early time-resolved experiments by Frauenfelder on the ensemble of the kinetic rebinding of CO to myoglobin molecules resulted in a stretched exponential relaxa-tion due to a very large spread of the reaction rates of individual molecules.
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Zebrafish as vertebrate model of Gaucher disease
Lysosomal storage disorders (LSDs) are a group of orphan diseases characterized by lysosomal dysfunction or impaired lysosomal catabolism and affect collectively about 1 in 5000 live births.
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Self adjuvanting immunopeptides: Design and synthesis
Chapter 2 describes a post-synthetic methodology to introduce a fluorescent label in highly lipophilic, Pam3Cys based conjugates.
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Another Brick in the Wall: The role of the actinobacterial cell wall in antibiotic resistance, phylogeny and development
Streptomyces are multicellular, Gram-positive bacteria in the phylum of actinobacteria which produce a high amount of bioactive natural products of which the expression is tightly coordinated with the life cycle.