In photoactivated chemotherapy (PACT), a biologically active compound is caged by a light-cleavable protecting group.

The research described in this thesis combines the latest insights in lysosomal function with lysosome centred cell signalling.

Hoe kan de techniek van cell wall engineering worden ingezet zodat de industrie streptomyceten beter kunnen verwerken tot nieuwe antibiotica?

In human cells, a meter-long DNA is condensed inside a micrometer-sized cell nucleus. Simultaneously, the genetic code must remain accessible for its replication and transcription to functional proteins.

The overarching goal of this thesis is to set the foundations, but also make the first essential steps towards establishing a comprehensive, mesoscopic, hydrodynamic theory of epithelial tissues. The stage is set by an exhaustive study of topological defects in passive p-atic liquid crystals, singularities…

Promotores: H.S. Overkleeft, G.A. van der Marel, Co-Promotor: R.G. Boot

Promotor: B. van de Water

Promotie

What is the mechanism of action preceding a physiological effects induced by metallic nanoparticles?

Promotor: J.G.E.M. Fraaije, Co-Promotor: A. Kros

Lysosomal acid glucosylceramidase (GBA1) is a lysosomal enzyme that degrades glucolipids with its main substrate being glucosylceramide (GlcCer). Defects in the GBA1 gene lead to glycosphingolipidosis Gaucher disease (GD), in which the hydrolysis of GlcCer is impaired and therefore, it accumulates in…

Pectin is a plant cell wall polysaccharide made of mainly D-galacturonic acid (GA) subunits. The potency of the filamentous fungus Aspergillus niger to naturally secrete high amounts of pectinases to degrade pectin has been utilized for the industrial production of pectinases.

Synthesis and application of chemical biology tools to study immunomodulatory signaling lipids.

In previous studies at our laboratory it was demonstrated that drug exposure of HepG2 cells can lead to an altered TNFα-induced NF-κB oscillatory phenotype, concurrent with a synergistically increased sensitivity for TNFα-induced apoptosis.

In this thesis, bioorthogonal chemistry is combined with correlative light-electron microscopy to selectively label and study pathogenic intracellular bacteria within the host immune cell.

Promotor: Roeland M.H. Merks

Promotores: Prof.dr. H. van Veen & Prof.dr. W. de Boer (Wageningen University)

All life on earth contains DNA, which is used to store biological information. Organisms compact their DNA in order for it to fit inside their cell(s).

The research described in this thesis focused on the use of bioorthogonal antigens to investigate immunological processes in antigen presenting cells. Bioorthogonal antigens are antigenic proteins produced through recombinant expression in a methionine auxotrophic E. coli strain.

Promotor: J. Kuiper

The type-IV secretion system (T4SS) is a machinery able to transfer DNA and proteins between bacteria and in certain cases also to eukaryotic cells.

Promotor: Johan Kuiper, Co-promotor: Saskia C.A. de Jager

The investigations described in this thesis lay out strategies aimed at advancing antibiotic research and development. The examples presented revolve around two main approaches: understanding drug-target interactions and target identification.

Studievoorlichting

Image-Pro Plus, ImageJ, Cell Profiler, R, Knime en NIS-elements

Nierfalen is een serieus probleem omdat nieren zichzelf niet kunnen regenereren na ernstige beschadiging. Een mogelijke oplossing bestaat uit het kunstmatig laten groeien van gezond nierweefsel. Daarvoor moeten wetenschappers eerst de ontwikkeling begrijpen tijdens de allereerste fase, in de foetus.…

What would be the environmental performance of the Carbon4PUR technology at industrial scale in the future?

Promotor: Thomas Hankemeier, Co-promotores: Heiko van der Linden, Paul Vulto

The function of TLRs in innate immunity has aroused worldwide attention soon after its discovery. Because of the broad functions of TLR2 in innate immunity, the drive for the development of TLR2-targeted vaccines or therapeutic treatments has accelerated in the last decades.

The applications of studying the early development of insects range from agriculture to material science.

Promotores: Prof.dr. W.J.G.M. Peijnenburg & Dr. M.G. Vijver

Promotores: A.H. Meijer, H.P. Spaink

In order to be able to develop effective medicine and treatments to prevent or cure autoimmune diseases or cancer we need to understand the mechanisms how they arise and what drives their course.Unravelling the fundamental molecular mechanisms influencing the onset and course of diseases such as allergies,…

This thesis describes the functional analysis of the Arabidopsis AHL15 gene.

Nanodiscs are small, disc-shaped structures designed to mimic biological cell membranes, composed of a lipid bilayer stabilized by scaffold proteins. Membrane proteins play crucial roles in essential processes such as nutrient and waste transport, molecular recognition, and cell signalling, making them…

Hoewel tuberculose tegenwoordig met antibiotica is te genezen, vormen nieuwe resistente varianten van de bacterie een steeds groter probleem. Bioloog Annemarie Meijer en haar collega’s onderzoeken de afweer tegen de ziekte. Dit biedt aanknopingspunten voor de ontwikkeling van medicijnen. 3 vragen aan…

Promotor: H.J.M. de Groot

Promotor: H.S. Overkleeft, Co-Promotor: B.I. Florea

This thesis describes the development of a variety of mannosylated conjugates. Antigen presenting cells bear mannoside recognizing receptors that actively transport antigen into the cell. This thesis exploits this feature for the development of improved vaccines.

Promotores: C.A.M.J.J. van den Hondel, V. Meyer, Co-promotor: A.F.J. Ram

Viral diseases constitute one of the major challenges in modern medicine. Membrane-targeting have broad-spectrum potential and could distinguish viral membranes from the host cell plasma membrane by the difference in membrane curvature.

In this thesis the combinatorial use of bioorthogonal labelling and Electron Microscopy (EM)-based imaging techniques is explored to enable observations of specific molecular targets in their ultrastructural context within the cell.

What is the environmental impact, the human health impact and the life cycle and production costs of the lab, pilot and commercial diffusion of nanowires and nanowire-based photovoltaic technologies?

Studievoorlichting

Prof.dr.ir. Lucas van Vliet is per 1 september 2012 aangesteld als hoogleraar Meerdimensionale beeldanalyse bij de faculteit Wiskunde en Natuurwetenschappen. Van Vliet blijft als hoofdfunctie zijn gelijknamige leerstoel bij de TU Delft bezetten.

eLaw onderzoekt de rol van het recht in de informatiemaatschappij: hoe kan het recht bijdragen aan een goede werking van informatie- en communicatietechnologie, en het gebruik ervan door burgers, bedrijven en overheden?