Synthesis and application of chemical biology tools to study immunomodulatory signaling lipids.

All life on earth contains DNA, which is used to store biological information. Organisms compact their DNA in order for it to fit inside their cell(s).

Promotores: Prof.dr. H. van Veen & Prof.dr. W. de Boer (Wageningen University)

Pectin is a plant cell wall polysaccharide made of mainly D-galacturonic acid (GA) subunits. The potency of the filamentous fungus Aspergillus niger to naturally secrete high amounts of pectinases to degrade pectin has been utilized for the industrial production of pectinases.

Lysosomal acid glucosylceramidase (GBA1) is a lysosomal enzyme that degrades glucolipids with its main substrate being glucosylceramide (GlcCer). Defects in the GBA1 gene lead to glycosphingolipidosis Gaucher disease (GD), in which the hydrolysis of GlcCer is impaired and therefore, it accumulates in…

The type-IV secretion system (T4SS) is a machinery able to transfer DNA and proteins between bacteria and in certain cases also to eukaryotic cells.

Promotor: Johan Kuiper, Co-promotor: Saskia C.A. de Jager

The investigations described in this thesis lay out strategies aimed at advancing antibiotic research and development. The examples presented revolve around two main approaches: understanding drug-target interactions and target identification.

Promotor: Roeland M.H. Merks

The research described in this thesis focused on the use of bioorthogonal antigens to investigate immunological processes in antigen presenting cells. Bioorthogonal antigens are antigenic proteins produced through recombinant expression in a methionine auxotrophic E. coli strain.

In previous studies at our laboratory it was demonstrated that drug exposure of HepG2 cells can lead to an altered TNFα-induced NF-κB oscillatory phenotype, concurrent with a synergistically increased sensitivity for TNFα-induced apoptosis.

In this thesis, bioorthogonal chemistry is combined with correlative light-electron microscopy to selectively label and study pathogenic intracellular bacteria within the host immune cell.

Promotor: J.G.E.M. Fraaije, Co-Promotor: A. Kros

Studievoorlichting

Image-Pro Plus, ImageJ, Cell Profiler, R, Knime en NIS-elements

Een overzicht van awards en prijzen die in 2019 zijn toegekend aan onze medewerkers en studenten, maar ook bijzondere benoemingen aan de Universiteit Leiden en andere instellingen.

Een overzicht van awards en prijzen die in 2021 zijn toegekend aan onze medewerkers en studenten, maar ook bijzondere benoemingen aan de Universiteit Leiden en andere instellingen.

What would be the environmental performance of the Carbon4PUR technology at industrial scale in the future?

Nierfalen is een serieus probleem omdat nieren zichzelf niet kunnen regenereren na ernstige beschadiging. Een mogelijke oplossing bestaat uit het kunstmatig laten groeien van gezond nierweefsel. Daarvoor moeten wetenschappers eerst de ontwikkeling begrijpen tijdens de allereerste fase, in de foetus.…

Promotor: Thomas Hankemeier, Co-promotores: Heiko van der Linden, Paul Vulto

The function of TLRs in innate immunity has aroused worldwide attention soon after its discovery. Because of the broad functions of TLR2 in innate immunity, the drive for the development of TLR2-targeted vaccines or therapeutic treatments has accelerated in the last decades.

The applications of studying the early development of insects range from agriculture to material science.

This thesis describes the functional analysis of the Arabidopsis AHL15 gene.

Promotores: Prof.dr. W.J.G.M. Peijnenburg & Dr. M.G. Vijver

Promotores: A.H. Meijer, H.P. Spaink

In order to be able to develop effective medicine and treatments to prevent or cure autoimmune diseases or cancer we need to understand the mechanisms how they arise and what drives their course.Unravelling the fundamental molecular mechanisms influencing the onset and course of diseases such as allergies,…

Nanodiscs are small, disc-shaped structures designed to mimic biological cell membranes, composed of a lipid bilayer stabilized by scaffold proteins. Membrane proteins play crucial roles in essential processes such as nutrient and waste transport, molecular recognition, and cell signalling, making them…

What is the environmental impact, the human health impact and the life cycle and production costs of the lab, pilot and commercial diffusion of nanowires and nanowire-based photovoltaic technologies?

Promotor: H.S. Overkleeft, Co-Promotor: B.I. Florea

This thesis describes the development of a variety of mannosylated conjugates. Antigen presenting cells bear mannoside recognizing receptors that actively transport antigen into the cell. This thesis exploits this feature for the development of improved vaccines.

Viral diseases constitute one of the major challenges in modern medicine. Membrane-targeting have broad-spectrum potential and could distinguish viral membranes from the host cell plasma membrane by the difference in membrane curvature.

Promotores: C.A.M.J.J. van den Hondel, V. Meyer, Co-promotor: A.F.J. Ram

In this thesis the combinatorial use of bioorthogonal labelling and Electron Microscopy (EM)-based imaging techniques is explored to enable observations of specific molecular targets in their ultrastructural context within the cell.

Promotor: H.J.M. de Groot

Studievoorlichting

Prof.dr.ir. Lucas van Vliet is per 1 september 2012 aangesteld als hoogleraar Meerdimensionale beeldanalyse bij de faculteit Wiskunde en Natuurwetenschappen. Van Vliet blijft als hoofdfunctie zijn gelijknamige leerstoel bij de TU Delft bezetten.

eLaw onderzoekt de rol van het recht in de informatiemaatschappij: hoe kan het recht bijdragen aan een goede werking van informatie- en communicatietechnologie, en het gebruik ervan door burgers, bedrijven en overheden?

Wiskunde en Natuurwetenschappen, Leiden Academic Centre for Drug Research (LACDR)

This thesis is about augmented reality (AR). AR is commonly considered a technology that integrates virtual images into a user’s view of the real world.

Studievoorlichting

In this thesis, we will utilize embryonic zebrafish tumour models to understand the interaction between engrafted human cancer cells and macrophages from the host, test drug administration modalities and anti-cancer efficacies of newly-developed PDT and PACT compounds, and test a light-triggered liposomal…

This thesis examines silicon pore optics (SPO), a technology that exploits silicon wafers from the semiconductor industry to create extremely high quality X-ray optics, by studying its manufacturing process, applications, and prospects.

Promotie

Promotie

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Pericytes, the mural cells of blood microvessels, are important regulators of vascular morphogenesis and function that have been postulated to mechanically control microvascular diameter through as yet unknown mechanisms.