Autopaghy is an intracellular degradation system which targets cytosolic components to lytic compartments for degradation and recycling of the building blocks of the cell.

The research described in this thesis combines the latest insights in lysosomal function with lysosome centred cell signalling.

This thesis focuses on the synthesis, characterization and performance towards CO2 electroreduction of mono and bi-metallic particles based on p-block metals

In this thesis, the discovery and optimization is described of chemical tools to study the N-acylethanolamine (NAE) biosynthetic pathway.

In this thesis, I targeted the orchid genus Lepanthes, one of the six genera of angiosperms that surpasses 1,000 species in the Neotropics, as a study model to investigate the evolutionary processes that promoted species diversifications.

In human cells, a meter-long DNA is condensed inside a micrometer-sized cell nucleus. Simultaneously, the genetic code must remain accessible for its replication and transcription to functional proteins.

Hoe kan de techniek van cell wall engineering worden ingezet zodat de industrie streptomyceten beter kunnen verwerken tot nieuwe antibiotica?

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“Temminck’s Order” is the scientific biography of Coenraad Jacob Temminck (1778–1850), a Dutch naturalist and the first director of ’s Rijks Museum van Natuurlijke Historie in Leiden.

The adenosine receptors are proteins that reside in the extracellular membranes of cells. Activation of adenosine receptors plays a role in many physiological and pathological processes, such as immune responses and cancers.

Wetenschappers van de Universiteit Leiden hebben een doorbraak bereikt in de bestudering van de erfelijke stofwisselingsziekte van Pompe. Samen met hun Yorkse collega’s ontwierpen zij een molecuul dat zich bindt aan het enzym dat aan de basis staat van het ziekteverloop. De bevindingen zijn gepubliceerd…

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Wiskunde en Natuurwetenschappen, Leids Instituut voor Onderzoek in de Natuurkunde (LION)

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Image-Pro Plus, ImageJ, Cell Profiler, R, Knime en NIS-elements

Hoogleraar Fons Verbeek, verbonden aan de informatica- en biologie-instituten van de Universiteit Leiden, heeft de fascinatie om alles wat we niet direct kunnen zien, toch in beeld te brengen. Op SleutelstadFM vertelt hij dat door het omrekenen van 2 dimensionale (2D) beelden naar 3 dimensionale (3D)…

In this project innovative technology for photovoltaics cells is developed and analysis of ecological and health related risks are explicitly accounted for.

What are the life-cycle environmental impacts and risks to human health and ecosystems of a III-V/Si PV system? How can these be expected to change when the system is deployed at industrial scale? What are the most favourable recycling scenarios?

What is the mechanism of action preceding a physiological effects induced by metallic nanoparticles?

Promotores: Prof.dr. H. van Veen & Prof.dr. W. de Boer (Wageningen University)

In previous studies at our laboratory it was demonstrated that drug exposure of HepG2 cells can lead to an altered TNFα-induced NF-κB oscillatory phenotype, concurrent with a synergistically increased sensitivity for TNFα-induced apoptosis.

In this thesis, bioorthogonal chemistry is combined with correlative light-electron microscopy to selectively label and study pathogenic intracellular bacteria within the host immune cell.

The molecular mechanisms that instigate a healthy cell to become malignant are fueled by (epi)genetic alterations in so-called driver genes.

Promotor: J. Kuiper

Promotores: A.H. Meijer, H.P. Spaink

Promotores: Prof.dr. W.J.G.M. Peijnenburg & Dr. M.G. Vijver

Promotor: Roeland M.H. Merks

Lysosomal acid glucosylceramidase (GBA1) is a lysosomal enzyme that degrades glucolipids with its main substrate being glucosylceramide (GlcCer). Defects in the GBA1 gene lead to glycosphingolipidosis Gaucher disease (GD), in which the hydrolysis of GlcCer is impaired and therefore, it accumulates in…

The investigations described in this thesis lay out strategies aimed at advancing antibiotic research and development. The examples presented revolve around two main approaches: understanding drug-target interactions and target identification.

The type-IV secretion system (T4SS) is a machinery able to transfer DNA and proteins between bacteria and in certain cases also to eukaryotic cells.

Pectin is a plant cell wall polysaccharide made of mainly D-galacturonic acid (GA) subunits. The potency of the filamentous fungus Aspergillus niger to naturally secrete high amounts of pectinases to degrade pectin has been utilized for the industrial production of pectinases.

This thesis describes the functional analysis of the Arabidopsis AHL15 gene.

Promotor: Johan Kuiper, Co-promotor: Saskia C.A. de Jager

The research described in this thesis focused on the use of bioorthogonal antigens to investigate immunological processes in antigen presenting cells. Bioorthogonal antigens are antigenic proteins produced through recombinant expression in a methionine auxotrophic E. coli strain.

All life on earth contains DNA, which is used to store biological information. Organisms compact their DNA in order for it to fit inside their cell(s).

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